Whole genome sequencing (WGS) for food-borne pathogen surveillance and control - Taking the pulse Academic Article uri icon


  • Next-generation sequencing (NGS) is transforming microbiology [1]. With the increased accessibility and decrease in the costs of sequencing and the optimisation of the 'wet laboratory'components of NGS ie the quality and throughput of DNA extraction, library preparation and sequencing reactions, whole genome sequencing (WGS) of bacterial isolates is rapidly revolutionising clinical and public health microbiology. WGS is a 'disruptive technology'that has the potential to become a one-stop-shop for routine bacterial analysis. By replacing multiple parallel steps in the microbiology diagnostic cycle, which currently involves traditional and molecular methods, it achieves accurate and speedy species identification, inference of antimicrobial susceptibility and virulence and highresolution subtyping [2]. Typing of food-borne pathogens was one of the earliest …

publication date

  • January 1, 2017