T-cell based sieve analysis ties HLA A* 02 to vaccine efficacy and IgA-C1 immune correlate in RV144 Thai trial Academic Article uri icon

abstract

  • Background The RV144 trial showed an estimated 31% vaccine efficacy (VE) against HIV-1 infection. Two immunological correlates of risk were found in vaccine recipients: Envelope V1/V2 antibody titers and IgA binding to Envelope (Haynes et al., 2012). Methods We conducted a CD8+ T-cell based sieve analysis of the V1/V2 region testing for differential escape from vaccine induced epitopes as predicted using computational methods. Breakthrough peptides that differed from predicted epitopes in the vaccine insert and had reduced HLA binding affinity were considered escapes. Results Three of twelve epitopes in V1/V2 of the MN protein boost showed evidence of escape (start-positions 147, 163, 168 restricted by A* 11 and A* 02), with more escapes in vaccine recipients (p= 0.018). We hypothesized that if escape was indicative of anamnestic responses, recipient's HLA …

authors

publication date

  • January 1, 2012