- The field of surface nanostructuring is growing rapidly with the need to search for more advanced fabrication solutions. The major challenge is the lack of appropriate combination of time/cost efficient techniques and medium possessing the advantages of both flexibility and tunable optical properties. Here we demonstrate direct-write femtosecond laser nanostructuring of indium–tin-oxide thin film where the deep-subwavelength ripples with periodicity of down to 120 nm are realized originating the form birefringence (|Δn| ≈ 0.2), which is 2 orders of magnitude higher than the commonly observed in uniaxial crystals or femtosecond laser nanostructured fused quartz. The demonstrated nanoripples with its continuously controlled space-variant orientation lead to the high density two-dimensional printing of flat optical elements. The technique can be extended to any highly transparent films that support laser-induced periodic surface structures, and can be effectively exploited for the integration of polarization sensitive modifications into multidimensional optical data storage.