63Nickel-constituents in renal cytosol of rats after injection of 63nickel chloride Academic Article uri icon

abstract

  • Chromatographic and electrophoretic fractionations of 63Ni-constituents were performed upon renal cytosol samples from rats that were killed following administration of 63NiCl2. At 0.5 to one hr after iv injection of 63NiCl2 (0.1 to 0.5 mumole per kg body wt., N= 6), renal cytosol contained 11 percent (SE+/-2 percent) of the 63Ni-dose. An average of 68 percent (SE+/-4 percent) of 63Ni in these cytosol samples was associated with low molecular weight components (mol. wt. less than 2,000). The remainder was bound to five macromolecular constituents, with molecular weights of greater than 130,000 (Peak A), approximately 70,000 (Peak B), approximately 55,000 (Peak C), approximately 30,000 (Peak D), and approximately 10,000 (Peak E), based upon column chromatography on Sephadex G-200. The 63Ni that was present in Peaks A to E averaged, respectively, 1.8 percent, 10.7 …

publication date

  • November 1, 1981