Using HLA binding prediction algorithms for epitope mapping in HIV vaccine clinical trials Conference Paper uri icon

abstract

  • Current HIV vaccines are designed to elicit both T-cell and B-cell responses. A common endpoint in any T-cell based vaccine trial are measurements of vaccine-induced T-cell responses such as breadth and magnitude [7]. In order to measure such endpoints blood samples are collected at multiple timepoints. Current immunological assays for measuring T- cell responses are functional assays in which peripheral blood mononuclear cells (PBMC) is incubated with target peptide (s) and then the release of various cytokines such as IFN-γ are measured. The major limiting factor in these mappping studies is sample availability, as each of these tests requires an order of 100K live cells. Therefore current mapping strategies use a group-testing approach in which responses to the immunogen are first measured using peptide pools that span a full protein, and are then further refined using sets of mini …

publication date

  • January 1, 2011