A comparison of genetic relationship measures in strawberry (Fragaria× ananassa Duch.) based on AFLPs, RAPDs, and pedigree data Academic Article uri icon

abstract

  • Nineteen of the major strawberry (Fragaria× ananassa Duch.) cultivars grown in the UnitedStates and Canada were examined for AFLP markerpolymorphisms. For the AFLP reactions, the Eco RI-ACC primer was used in combination with four Mse I primers (Mse I- CAC, Mse I-CAG, Mse I-CAT, or Mse I-CTT). Each set ofprimers produced 46–66 scorable fragments ranging insize between 50 and 500 bp. The polymorphic fragmentsproduced from each set of primers were more thansufficient to distinguish among all the cultivars, demonstrating the usefulness of AFLP markers forcultivar identification. Similarity coefficients werecalculated based on data from 228 AFLP markers anddata from 15 previously characterized RAPD markers. The RAPD markers had been specifically selected forfingerprinting purposes because they succesfullydistinguish 41 strawberry cultivars …

publication date

  • January 1, 2001