Differential Phosphorylation in the Motor Domain of Mitotic Kinesin-5 Cin8 Regulates its Functions In Vivo Academic Article uri icon

abstract

  • Cin8, a kinesin-5 mitotic motor protein in S. cerevisiae, moves directionally along microtubules (MTs) by hydrolyzing ATP. Kinesin-5 mitotic motors exhibit tetrameric structure that enables them to crosslink two MTs, thus performing their essential roles in mitotic spindle formation and elongation. It was previously demonstrated in our lab, that Cin8 is differentially phosphorylated during anaphase in one or more of the three cyclin-dependent kinase 1 (Cdk1) sites located in its catalytic domain: S277, T285 and S493.

publication date

  • January 1, 2018