Distribution of blood glutamate into peripheral tissues is increased by stress response and adrenaline administration, providing neuroprotection following traumatic b... Academic Article uri icon

abstract

  • Materials and Methods: 48 Sprague Dawley anesthetized rats were divide into treatment groups: Sham, AD, ME, PR, Pyruvate, Oxaloacetate. Treatments were infused intravenously for 30 min at 1μg/100g/min. Rats were then injected with radiolabeled G 1ml= 10μci L-[G-3H] glutamic acid per rat intravenously. Arterial blood samples were collected every 2.5 min for10 min. Rats were sacrificed and the peripheral organs, including skeletal muscle, were removed and weighed, and 0.1 g tissue samples were obtained. Tissue samples were incubated and dissolved in 1 ml solvent, and their radioactivity measured. Radioactivity of muscle tissue, relative to the total body mass was calculated, and groups compared. Results and Discussion: Muscle tissue accumulated the majority of radiolabeled G from the blood at 10 min following injection (54–69%). Accumulation was significantly increased in the AD …

publication date

  • June 12, 2010