Next-generation sequencing identifies and immunohistochemistry confirms a novel crizotinib-sensitive ALK rearrangement in a patient with metastatic non-small-cel... Academic Article uri icon


  • Panel A, Using a manual fluorescent in situ hybridization technique, paraffin sections were hybridized with fluorescent ALK Break-Apart probe (Abbott Molecular, Des Plaines, IL). The specimen displayed an atypical pattern of double 3′ALK signals (red) fused with the 5′ALK signal (green), which was classified as negative for ALK gene rearrangement (< 15% of cells with split signals, 3′ ALK and 5′ ALK signals apart by >2 times signal size or single 3′ ALK signal, 50 cells scored). Panels B, C, and D, ALK immunohistochemistry was performed using a primary antibody (clone D5F3, Cell Signaling Technology, Inc, Danvers, MA). All paraffin tissue sections were stained with ALK and were reviewed by two pathologists. The tumor cells have predominantly strong staining (3+) in 50% of the tumor cells (lower right). The remaining tumor cells showed (2+) in 30% (lower left) and (1+) in 20% of the … A, Hypothetical structure of …

publication date

  • September 1, 2012