- In summary, the presence of hidden autoantibodies in the sera of healthy people is established 151. They are detected by separating the immunoglobulin from the whole serum, heating or adding various chemical components that alter the physiological features of the immunoglobulin. It is plausible that exposure to environmental antigens leads to a change in the physiological characteristics of the blood and unmasks the autoantibody activity of hidden auto-antibodies. Hidden auto-antibodies bind a wide range of self-antigens; however, they are not pathogenic and cannot be used in screening strategies for early diagnosis of auto-immune diseases. Solid evidence supports a role for natural auto-antibodies in biological and physiological regulation.