New synthetic models of cytochrome P450: How different are they from the natural species? Academic Article uri icon

abstract

  • Soluble and matrix-bound P450 enzyme models have been synthesized carrying a SO 3 - ligand coordinating to iron. These complexes display features very similar to cofactors of enzymes such as P450 c a m with respect to electrochemistry and UV/Vis spectroscopy. Further they catalyze epoxidation reactions with turnover numbers up to 1800. DFT calculations revealed that the coordination of SO 3 - to Fe(III) produces an active species that displays allylic hydroxylation and epoxidation reactivity patterns that are nearly indistinguishable from those calculated for the natural active species of the enzyme cytochrome P450.

publication date

  • January 1, 2005