- Abstract The fatty acid distribution in 19 strains of Spirulina was studied. All but one contained γ-linolenic acid (GLA). No GLA was found in S. subsalsa , which had a very high content of palmitoleic acid. The fatty acid content of all but one of the tested strains increased with cultivation temperature and the relative amount of polyunsaturated fatty acid decreased. The highest content of GLA was found at 30–35° for most strains. High light intensities at a high temperature (38°), while not affecting the fatty acid composition, had a drastic effect on the fatty acid content, reducing it by as much as 46 %.