- Liquid crystal (LC) devices are widely used as building blocks of many electro-optical systems including linear polarization rotators, dynamical wave plate retarders, and pixilated devices for displays, spatial light modulators, and tunable filters. Precise alignment of the LC molecules is required for high quality components. The anisotropic nature of LC molecules allows them to align on solid surfaces. This can be achieved either due to physicochemical interaction such as photo-alignment on surfaces using polarized blue light or due to the elastic interaction when aligned along nanogrooves created by mechanical rubbing or lithography techniques. Although numerous methods enabling the manufacturing of LC devices have been reported, the technological flexibility and precision remains a problem. We propose a direct-write ultrafast laser nanostructuring as an alternative method capable of fabricating anisotropic surface elements. One of the most fascinating aspects of this technique is the ability to induce tunable structures with subwavelength periodicities that generate form birefringence. Such engineered surfaces can be implemented as a host substrate for controlled organization of various materials such as LC molecules. In this study we demonstrate the LC alignment on femtosecond laser nanostructured ITO coated glass. Nine regions of laser-induced nanostructures are depicted. Each column was fabricated under different pulse energy, while the orientations of the anisotropy at each row was changed from horizontal to diagonal and to vertical in order to achieve different alignment directions.