HUMAN T LYMPHOCYTE ACTIVATION BY TUMOR PROMOTERS: PROTEIN KINASE C' Academic Article uri icon

abstract

  • MATERIALS AND METHODS Reagents. Ficoll-Hypaque. dimethylsulfoxide (DMSO), mezerein, phorbol esters, phenylmethylsulfonyl fluoride (PMSF), ethylenediaether) NN, N'. N" tetraacetic acid (EGTA). bovine serum albumin. H1 mine tetraacetic acid (EDTA), ethyleneglycol-bfs-(j3-amino-ethyl-phatidylserine [PS) were from Sigma Chemical Co.[St. Louis. MO). histone type 111-S. 1.2-diolein. trichloroacetic acid, ATP, and phos-Triton X-100 and Tris were from Bio-Rad Laboratories (Richmond, CA). DE-52 ion exchange cellulose and GF/F filters were from Whatman (Maidstone, UK). and [r-32P] ATP (specific activity 7000 Ci/mmol) was from ICN Radiochemicals (Irvine, CA). Neuraminidase was from GIBCO (Grand Island, NY), CPZ was from Elkins-Sinn (Cherry Hill. NJ). ionomycin was from Calbiochem (La Jolla, CA), and phytohemagglutinin was from Wellcome Diagnostics …

publication date

  • January 1, 1767