Insulin-like gene in prawn sexual differentiation Conference Paper uri icon

abstract

  • Androgenic glands (AGs) are unique endocrine glands regulating male sexual differentiation and growth in crustaceans. In the prawn Macrobrachium rosenbergii complete sex reversal was achieved by AG grafting or removal in females and males, respectively. In the prawn M. rosenbergii AG cDNA subtractive library was constructed. Screening of this library revealed an AG specifically expressed gene, termed M. rosenbergii insulin-like AG factor (Mr-IAG). The predicted mature Mr-IAG peptide (B and A chains) showed high similarity in its cysteine backbone to other crustacean AG specific insulin-like peptides with low sequence homology. Mr-IAG was found to have a prominent expression comprising 55% of the library sequences. Using real-time RT-PCR Mr-IAG transcript level was found to be higher in the sexually active morphotypes relative to its level in the sexually less active morphotype. In vivo, Mr-IAG dsRNA injections …

publication date

  • February 1, 2009