Towards Single Cell Optical Tomography Academic Article uri icon

abstract

  • 3132-Plat Towards Single Cell Optical Tomography Mario Feingold, Gideon Carmon, Itzhak Fishov. Ben Gurion University of the Negev, Beer Sheva, Israel. Using a single-beam, oscillating Optical Twee- zers we demonstrate trapping and rotation of rod-shaped bacterial cells with respect to the op- tical axis [1]. The angle of rotation is deter- mined by the amplitude of the oscillation. This technique allows imaging fluorescently la- beled 3D sub-cellular structures from different, optimized viewpoints. To illustrate our method we analyze the Z-ring of E. coli [2]. We use cells that express FtsZ-GFP and have their cyto- plasmic membrane stained with FM4-64 (Fig- ure). In a vertically oriented cell, both the Z- ring and the cytoplasmic membrane images ap- pear as symmetric circular structures that lend themselves to quantitative analysis. Scanning the cell alignment and using 3D image reconstruction from the corresponding images of …

publication date

  • January 31, 2012