- Abstract The optimal method for surveillance of carbapenem-resistant Acinetobacter spp.(CRAB) is unknown. A collection of CRAB strains (n= 42), carbapenem-susceptible strains (CSAB), and non-Acinetobacter strains (n= 18) was used to evaluate six laboratory surveillance methods: MacConkey (MAC), MAC+ 1 μg/ml imipenem (MAC-IPM), minimal salts agar+ 1% acetate (MSA), MSA with IPM disk (MSA-IPM), CHROMagarKPC, and CHROMagar Acinetobacter with CR102 (CHROMAcineto). CHROMAcineto was 100% sensitive and specific. CHROMagarKPC and MAC-IPM were highly sensitive (> 95%), but their specificity was substantially hampered by the breakthrough growth of CSAB. MSA was unsuitable for CRAB detection. CHROMAcineto is a promising medium for CRAB detection and warrants further clinical evaluation.