Ovarian protein synthesis in the prawn macrobrachium rosenbergii: Does ovarian vitellin synthesis exist? Academic Article uri icon

abstract

  • Summary During the reproductive cycle oocytes of Macrobrachium rosenbergii females grow in diameter from 20 μm to 650 μm, while the gonado-somatic index increases from 0.2 to 8.0. SDS-PAGE polypeptide profiles of the cytosolic fraction from ovaries in various stages were studied. Specific vitellin bands were identified by immunoblotting. In vitro synthesis of protein was measured by TCA precipitation and autoradiography of SDSPAGE separated polypeptides. Incorporation of [35S]-methionine and cysteine was highest in animals having an oocyte diameter of 50–150 pm and lower in pre-vitellogenic ovaries (20–50 μm). The lowest synthesis was found in late-vitellogenic ovaries (300–500 μm). Most of the incorporation was found in non-vitellin polypeptide bands. These results suggest the existence of an extraovarian source of vitellogenin in M. rosenbergii.

publication date

  • January 1, 1995