In-Vitro Percutaneous Studies with 5% Acyclovir Cream: Histopathological evidence for time-dependant diffusivity Academic Article uri icon

abstract

  • Percutaneous drug penetration studies were performed, during which skin properties were altered. The present research characterizes, in particular, the in vitro permeation kinetics of a 5% acyclovir cream through hairless mouse skin. Cumulative amounts of penetrated acyclovir per 1 cm2 area were monitored in a Franz diffusion cell system. Statistical models were constructed to interpolate the experimental data obtained from 11 sets of experiments (each, n= 6). The in vitro penetration of acyclovir through hairless mouse skin over a 24-h period was found to be non-linear, with a slow" early phase" up to 12 hours, followed by a rapid" late phase"(approximately 10 times faster). The histological finding of epidermal necrosis in the latter periodbut not in the first 12 hoursprobably explains the non-linear elevation of the drug transport rate in the diffusion study. The data presented in this paper …

publication date

  • January 1, 2001