The role of Cell-free DNA as a marker to predict acute liver injury in rats: 12AP5-1 Academic Article uri icon


  • Materials and Methods: ALI was induced in 45 rats by carbon tetrachloride, administered by a nasogastric tube at doses of 1 ml/kg, 2.5 ml/kg, or 5 ml/kg of 50% solution. Fifteen additional rats were given a sham procedure. Blood samples were taken at t= 0 (baseline), 3, 6, 12, 24, 48, 72, 96 and 120 hours for measurements of CFD, GPT, GOT, and total bilirubin. Histology and prothrombin time were examined at 24 and 120 hours after injection of 5 ml/kg carbon tetrachloride in 18 additional rats and in 10 control rats. Results and Discussion: CFD levels in rats following ALI were significantly elevated in all blood samples starting at 12 hours after ALI (P< 0.001). Levels of blood GPT, GOT and total bilirubin were increased in all blood samples starting at 3 hours after ALI (P< 0.0001). CFD levels correlated with GPT (R 2= 0.92), GOT (R 2= 0.92) and total bilirubin (R 2= 0.76). There …

publication date

  • June 1, 2013