The hepatopancreas as a site of yolk protein synthesis in the prawn macrobrachium rosenbergii Academic Article uri icon

abstract

  • Summary Our previous study failed to show vitellin synthesis in the ovary of the prawn Macrobrachium rosenbergii (Sagi et al., 1995); thus the role of the hepatopancreas as a possible site of synthesis was evaluated. Extracts of hepatopancreas and hemolymph of a secondary-vitellogenic female exhibited higher levels of yolk protein than those from a primary-vitellogenic female. Clear vitellin immuno-cross-reactivity was observed in hepatopancreas sections from a secondary-vitellogenic female while no such reaction was found in a male hepatopancreas. Furthermore, vitellin-immuno-cross-reactive polypeptides released into the culture medium of the hepatopancreas of a secondary-vitellogenic female were similar to those found in the hemolymph and ovary (92 and 105kDa). The most prominent immuno-reactive polypeptide in the hepatopancreas extract was a relatively low-molecular-weight species (42kDa). De novo synthesis of cross-reactive-vitellin polypeptides (34, 38 and 42kDa) was detected in the hepatopancreas of...

publication date

  • January 1, 2000