DNA-Based Vaccines: Role of Dendritic Cells in Antigen Presentation Academic Article uri icon

abstract

  • Since the discovery that plasmid DNA encoding a protein antigen can serve as an effective immunogen, DNA-based vaccination has become an attractive alternative to recombinant live vector immunization strategies. DNA vaccination has several obvious advantages over traditional vaccines (protein and peptide-derived), namely, more safely, easier to manufacture and to manipulate the protein antigenicity in the level of DNA. Moreover, the addition of DNAs encoding various cytokines or costimulatory molecules admixed with DNA encoding antigens serves to enhance the magnitude and type of desired immune responses (Xiang and Ertl 1995). In different animal models, DNA vaccines have been found to be protective against a wide range of diseases, including cancers, infectious, autoimmune and allergic diseases (Tighe et a1 1998). The question arises why is DNA immunization so effective at inducing specific protective immunity? Considering of some features of DNA vaccine provides the answer to this question: (i) it provides an immunogenic antigen; (ii) it is processed via major histocompatibility complex (MHC) class I and II; (iii) it stimulates immunological memory; and (iv) it contains an adjuvant (Tighe et al

publication date

  • January 1, 2002