The role of adrenoreceptors in the regulation of blood glutamate level in a brain self-protecting mechanism after closed head injury Academic Article uri icon


  • Materials and Methods: After approval of the Animal Care Committee, spontaneously breathing 150 male Sprague Dawley rats weighing 200–300 g were anesthetized with 2% isoflurane and randomly assigned to groups within one of two cohorts. In one cohort without CHT (n= 48) the treatment groups were: 1 sham, 2-imitation of surgical stress induced by craniotomy, 3-adrenaline 10 ug/kg/min for 30 min, 4-noradrenaline 20 ug/kg/min for 30 min, 5-isoproteronol 50 ug/kg ip, 6-phenylephrin 30 ug/kg/min within 30 min, 7-imitation of surgical stress by craniotomy+ propranolol 10 mg/kg ip, 8-metaprolol 15 mg/kg ip. Rats were treated immediately after collecting a baseline blood G sample and blood was further collected at 60, 90 and 105 min following CHT. In a second cohort with CHT (n= 56), rats were pretreated at sixty min before CHT and assigned to one of three groups: 9-CHT with …

publication date

  • January 1, 2006