Superoxides produced by angiotensin II-stimulated phagocytes mediate the adhesion of phagocytes to endothelial cells Academic Article uri icon

abstract

  • Our previous results demonstrate an immediate activation of NADPH oxidase in angiotensin II-stimulated phagocytes as well as in differentiated PLB-985 cells. The proposed study intends to determine the effect of superoxide radicals produced by angiotensin II-stimulated phagocytes on their adhesion to endothelial cells. We established an in vitro co culture model of granulocyte-like PLB-985 or X-linked chronic granulomatus disease (X-CGD) PLB-985 cells with human EC in direct contact. Adhesion was measured by flow cytometry analysis. The cells were allowed to bind to EC for different periods of time at 37°C in a 5% CO2 incubator. After mild trypsinization and several washes, the cells were incubated with anti CD-11b and then analyzed by flow cytometry. Similar to PLB-985 cells, X-CGD PLB-985 cells differentiated to granulocytic lineage but failed to activate NADPH-oxidase in response to angiotensin II. Adhesion of both granulocyte-like PLB-985 cells and X-CGD PLB-985 cells to endothelial cell was detected after 15 min and was maximal after 1 hour. Addition of 10−6M angiotensin II to the co-culture medium enhanced the adhesion of granulocyte-like PLB-985, however it did not affect the adhesion of granulocyte-like X-CGD PLB-985 cells to endothelial cells. These results suggest that superoxides generated by angiotensin II-stimulated granulocyte mediate the adhesion of granulocytes to endothelial cells. Am J Hypertens (2004) 17, 242A–243A; doi: 10.1016/j.amjhyper.2004.03.649

publication date

  • January 1, 2004