MOLECULAR BASIS OF CELL AND DEVELOPMENTAL BIOLOGY-Two phases of chromatin decondensation during dedifferentiation of plant cells. Distinction betw... Academic Article uri icon

abstract

  • Abstract Cellular dedifferentiation is the major process underlying totipotency, regeneration, and formation of new stem cell lineages in multicellular organisms. In animals it is often associated with carcinogenesis. Here, we used tobacco protoplasts (plant cells devoid of cell wall) to study changes in chromatin structure in the course of dedifferentiation of mesophyll cells. Using flow cytometry and micrococcal nuclease analyses, we identified two phases of chromatin decondensation prior to entry of cells into S phase. The first phase …

publication date

  • January 1, 2001