Enhancement of eicosapentaenoic acid (EPA) and γ-linolenic acid (GLA) production by manipulating algal density of outdoor cultures of Monodus subterraneus (Eu... Academic Article uri icon


  • The effect of algal density on cell growth and composition with special reference to the production of eicosapentaenoic acid (EPA) and γ‐linolenic acid (GLA) was investigated in semi-continuous outdoor cultures of Monodus subterraneus and Spirulina platensis. In both species, an exponential decrease in specific growth rate and a positive skewed pattern in biomass productivity were associated with an increase in density. In M. subterraneus, the highest EPA cell content (3· 8% of dry weight) occurred at the optimal density of c. 4gl− 1 ie the density that yields the highest output rate of biomass per culture volume or area. In S. platensis, high density was associated with a decrease in total fatty acid content, but the relative abundance of GLA increased. GLA content was therefore fairly stable at c. 1% of dry weight throughout the range of algal density tested. Fatty acid desaturation seemed to be …

publication date

  • February 1, 1997