Alginate scaffold for organ culture of cryopreserved-thawed human ovarian cortical follicles Academic Article uri icon

abstract

  • Purpose To compare macroporous alginate scaffolds with Matrigel for culturing frozen- thawed human primordial follicles in organ culture. Methods Twelve girls/women donated ovarian tissue. One tissue sample was fixed immediately after thawing (uncultured samples). Slices were cultured for 2 weeks on either Matrigel or on alginate scaffolds with a serum-free culture medium. Growth evaluation consisted of follicular counts and classification, immunohistochemistry and measurement of 17β-Estradiol (E 2) production. Results The number of developing follicles was significantly higher in alginate scaffold-cultured samples than on Matrigel with a concomitant decrease in the number of primordial follicles in alginate scaffold-cultured samples than uncultured samples. The number of atretic follicles after 1 week was significantly higher in the Matrigel-cultured samples than in the alginate …

publication date

  • January 1, 2011