Polymeric carrier-mediated intracellular delivery of phosphatidylinositol trisphosphate to overcome insulin resistance Conference Paper uri icon

abstract

  • Background and aims: Production of phosphatidylinsoitol-3, 4, 5-trisphosphate (PIP3), induced by insulin is a major signaling step in the induction of insulin's metabolic actions in muscle and fat, and is impaired in various states of insulin resistance. Intracellular delivery of PIP3 into cells might therefore constitute an approach for bypassing such a signaling defect. However, this approach is challenged by the need to overcome permeability barriers through the plasma membrane, and to introduce the agent in well-defined spatial-temporal coordinates corresponding to endogenous PIP3 generation. Exogenous PIP3 may be introduced into cells using polycationic carriers which should mask its negative charges. However so far, these approaches only partially mimicked insulin's effects in normal, insulin sensitive cells, and displayed poor efficiency and low reproducibility. The overall aim of …

publication date

  • January 1, 2008