Oxaloacetate-treatment caused a significant reduction of brain water content after closed head trauma Academic Article uri icon


  • Materials and methods: After approval of the Animal Care Committee, spontaneously breathing 24 male Sprague Dawley rats weighing 200–300 g were anesthetized with 2% isoflurane and a cranial impact of 0.5 J was delivered by a rod. Rats were injected intravenously for a total duration of 30 min with OxAc (30μmoles/min/100 g) or saline 0.9% 1 ml/100 g. Brain hemispheres were removed 120 min after TBI in some groups while in others, brain tissue samples of approximately 50 mg were excised at 24 h post TBI from a location immediately adjacent to the area of macroscopic damage in the left hemisphere and from a corresponding area in the right hemisphere. These tissue samples were used for determination of water content. Water content was determined from the difference between wet weight (WW) and dry weight (DW). Specifically, after WW of fresh brain tissue …

publication date

  • January 1, 2006