High fidelity reconstruction of three-dimensional objects by FINCH fluorescence microscopy Conference Paper uri icon

abstract

  • 1Department of Biomedical Engineering, Johns Hopkins University, 9605 Medical Center Drive, Rockville, Maryland 20850 USA 2Microscopy Center, Johns Hopkins University Montgomery County Campus, Rockville, Maryland 20850 USA 3 Department of Electrical and Computer Engineering, Ben-Gurion University of the Negev, PO Box 653, Beer-Sheva 84105, Israel *gbrooker@jhu.edu … Abstract: FINCH holography produces super-resolved images above and below the focal plane of the microscope objective but with different magnifications. A method is presented to faithfully reconstruct 3D objects with dimensional fidelity. © 2012 Optical Society of America … OCIS codes: (090.1760) Computer holography; (100.6890) Three-dimensional image processing; (110.0180) Microscopy;; (180.2520) Fluorescence microscopy; (180.6900) Three-dimensional microscopy. (090.1995) Digital holography.

publication date

  • January 1, 2012