NCLX is an essential component of mitochondrial Na⠺/Ca²⠺ exchange Academic Article uri icon

abstract

  • Abstract Mitochondrial Ca²⠺ efflux is linked to numerous cellular activities and pathophysiological processes. Although it is established that an Naâ º-dependent mechanism mediates mitochondrial Ca²⠺ efflux, the molecular identity of this transporter has remained elusive. Here we show that the Naâ º/Ca²⠺ exchanger NCLX is enriched in mitochondria, where it is localized to the cristae. Employing Ca²⠺ and Naâ º fluorescent imaging, we demonstrate that mitochondrial Naâ º-dependent Ca²⠺ efflux is enhanced upon overexpression of NCLX, is reduced by silencing of NCLX expression by siRNA, and is fully rescued by the concomitant expression of heterologous NCLX. NCLX-mediated mitochondrial Ca²⠺ transport was inhibited, moreover, by CGP-37157 and exhibited Liâ º dependence, both hallmarks of mitochondrial Naâ º-dependent Ca²⠺ efflux. Finally …

publication date

  • January 1, 2010