Effect of Propranolol and IFN-β on the Induction of MHC Class II Expression and Cytokine Production by IFN-γ in THP-1 Human Monocytic Cells Academic Article uri icon

abstract

  • Abstract This study was undertaken to investigate the effects of propranolol, IFN-β, and the protein kinase modulators on IFN-γ induction of MHC class II antigen expression and cytokine production in THP-1 human monocytic cells. IFN-γ induced expression of HLA-DR and DQ molecules and secretion of the monokines IL-1β and TNF-α in THP-1 cells in a time and dose-dependent manner. The effect of IFN-y on class II HLA antigens was dose- dependently inhibited by IFN-β. H-7, phloretin, staurosporine as well as GF 109203X are selective enzyme inhibitors of protein kinase C (PKC), down-regulating IFN-γ induced MHC class II expression and cytokine production. Stimulators of PKC, like PMA, replaced IFN-γ in the induction of monokines in THP-1 cells, whereas the addition of HA 1004 or arachidonic acid to the culture had no effect on IFN-γ mediated changes. Blocking of phospholipase D …

publication date

  • January 1, 1998