Mapping of the human protein kinase C-θ (PRKCQ) gene locus to the short arm of chromosome 10 (10p15) by FISH Academic Article uri icon

abstract

  • Members of the protein kinase C (PKC) family of serine/threonine kinases play critical roles in the regulation of cellular differentiation and proliferation of diverse cell types. In an attempt to find PKC isoforms that are involved in growth control and/or activation of T lymphocytes, we have used a human peripheral blood lymphocyte-derived cDNA library to identify a novel PKC isoform, termed PKC-{theta}. The human PKC-{theta} cDNA (human gene symbol PRKCQ) was characterized and found to encode an {approximately} 80-kDa protein, expressed predominantly in lymphoid tissues and hematopoietic cell lines, in particular T cells. A murine homolog gene derived from skin cDNA library was found to be expressed predominantly in skeletal muscle. Molecular cloning and biochemical studies identified PKC enzymes as members of a distinct family that constitutes, at the gene level, nine mammalian members, i.e., {alpha}, {beta}, {gamma}, {delta}, {epsilon}, {zeta}, {theta}, and {lambda}, of which three have already been assigned to individual human chromosomes. Thus, PKC-{alpha} was mapped to H17q22-q24, PKC-{Beta} to H16p12-q11.1, and PKC-{gamma} to H19q13.4. The chromosomal location of other members is yet unknown. 13 refs., 1 fig.

publication date

  • January 1, 1995