591: Maternal total cell free DNA in preeclampsia and IUGR: evidence for differences in maternal and fetal response for abnormal implantation Academic Article uri icon

abstract

  • Objective Preeclampsia (PE) and IUGR are major obstetrical syndromes associated with abnormal placental implantation and changes in the activation status of maternal leucocytes. This study is aimed to determine the changes in maternal serum total cell-free DNA (t- cfDNA) concentrations, measured by a simple rapid fluorescent assay, in women with preeclampsia and in those with fetal growth restriction. Study Design A cross sectional study was conducted measuring maternal serum t-cfDNA concentrations. Women were classified into the following groups: 1) patients with PE (n= 21); 2) IUGR (n= 28); and 3) normal pregnancy (n= 39). Serum samples were directly assayed for t-cfDNA using a rapid fluorescent SYBR Gold stain, without prior DNA extraction and amplification. Elevated maternal serum t-cfDNA concentrations were defined as a cutoff> 850ng/ml …

publication date

  • January 1, 2016