Protein Structure Alteration Induced by Light-Activated Water Absorption. A Study with Bacteriorhodopsin Academic Article uri icon

abstract

  • Water is a crucial component in determining the structure of proteins. Nonetheless, it has been difficult to address the problem of the role of water in protein structure in terms of both experiments and theory. Therefore, the general understandings that have been developed concerning the structure of proteins have emphasized the sequence of amino acids as the sole determinant in the structure of proteins. In this paper the development of a system is reported in which light regulates the amount of water that is absorbed by a protein structure. The system we report in this paper is the ligand binding reaction of the protein bacteriorhodopsin. 1 The ligand is a retinal chromophore characterized by an all-trans configuration, covalently bound to the protein via a protonated Schiff base. 2 It is known that 9-cis-retinal binds with the apoprotein bacterioopsin. Nevertheless, formation of the …

publication date

  • January 1, 1996