558: Cannabidiol enhances placental permeability to xenobiotics throughout direct inhibition of breast cancer resistance protein in the human placental barrier Academic Article uri icon

abstract

  • Objective Illicit drugs affect pregnancy outcome, however, the mechanisms in which cannabis exert its effect is not well understood. The aims of our study are to examine the effect of acute exposure (1-2 hours) to cannabidiol (CBD) on human placental Breast Cancer Resistance Protein (BCRP) function in in-vitro and ex-vivo models. Study Design CBD impact on BCRP in BeWo and Jar cells was tested with Mitoxantrone (MX)(BCRP substrate) uptake assay, using Nicardipine (BCRP inhibitor) as a positive control. CBD impact on BCRP function in human placenta was examined by testing Glyburide transport (known BCRP substrate) across term, normal, human cotyledon that was dually perfused for 2 hours, with [CBD group, n= 5] or without [control group, n= 5] CBD. Glyburide was introduced to maternal (M) and fetal (F) compartments, and F/M ratio of its concentrations …

publication date

  • January 1, 2013