- maternal serum anti-genital mycoplasma antibodies, and preterm parturition Offer Erez, Ruthy Beer Weisel, Sari Prutchi Sagiv, Tal Rafaeli, Ilan Levi, Vered Klaitman, Barak ArichaTamir, Alona Kuzmina, Limor Besser, Orna Staretz-Chacham, Iris Shoham, Michela Quaranta, Gershon Holcberg, Moshe Mazor Soroka University Medical Center, School of Medicine, Faculty of Health Sciences, Ben Gurion University of the Negev, Department of Obstetrics and Gynecology, Beer Sheva, Israel, Soroka University Medical Center, School of Medicine, Faculty of Health Sciences, Ben Gurion University of the Negev, Department of Neonatology, Beer Sheva, Israel, Soroka University Medical Center, Promyco Diagnostics, Beer Sheva, Israel, Clalit Health Services, Mor Research Applications, Tel Aviv, Israel, Policlinico GB Rossi Azienda Ospedaliera, Obstetrics and Gynecology Departement, Verona, Italy OBJECTIVE: Genital mycoplasmas (GM) are the most prevalent intraamniotic microorganism in women with preterm parturition. In contrast, the relationship between cervical colonization with these microorganisms and preterm parturition is not well established. The aim of this study was to determine the association between maternal serum concentrations of anti GM antibodies (AB) and spontaneous preterm delivery (PTD). STUDY DESIGN: a prospective observational study was conducted including women with preterm parturition (n 131) and patients in labor at term (n 22) as controls. Maternal serum AGM-AB were determined by the PROMYCO kit a novel immunoassay. RESULTS: There was a positive correlation between maternal serum AGM-AB during the episode of preterm parturition and at delivery (r 0.92, p 0.0001). Median maternal serum AGM-AB was higher in women with a positive GM cervical culture than in those with a negative one (p 0.001). There was no correlation between gestational age at sample collection and maternal serum AGM-AB concentrations. Women with preterm parturition who delivered preterm and a positive cervical GM culture had a higher rate of elevated AGM-AB ( 75th percentile) than those with a sterile cervical culture (83.3% vs. 41.5%, p 0.012). Among women with preterm labor and a positive GM cervical culture, those who delivered preterm had a higher median AGM-AB than those who delivered at term (p 0.042). CONCLUSION: Maternal cervical colonization with GM is associated with elevated serum AGM-AB concentrations. In the presence of cervical colonization with genital mycoplasma, maternal serum concentrations of AGM-AB may assist in identifying those who will deliver preterm following an episode of preterm parturition. This is important, since our observation might contribute to a better identification of patients with cervical colonization with GM and preterm parturition that may benefit from antimicrobial treatment.