400: Maternal total cell-free DNA in different phenotypes of the preterm parturition syndrome-preterm labor and preterm PROM Academic Article uri icon

abstract

  • Objective To determine whether the concentrations of circulating total cell-free DNA (t- cfDNA) in maternal serum, measured by a simple rapid fluorescent assay, are associated with the two phenotypes of spontaneous preterm parturition syndrome (preterm labor and preterm PROM). Study Design A cross-sectional study was conducted measuring maternal serum t-cfDNA concentrations. Women were classified into the following groups: 1) term non- labor (n= 21); 2) term labor (n= 15); 3) preterm non-labor (n= 18); 4) preterm labor (n= 25); and 5) preterm PROM (n= 14). Serum samples were directly assayed for t-cfDNA using a rapid fluorescent SYBR Gold stain, without prior DNA extraction and amplification. Elevated maternal serum t-cfDNA concentrations were defined as a cutoff> 850 ng/ml. Nonparametric statistics were used for analysis. Results In both non-labor groups, t-cfDNA …

publication date

  • January 1, 2016