INITIAL STUDIES ON THE FATTY ACID DESATURASE GENES IN THE UNICELLULAR GREEN ALGAE PARIETOCHLORIS INCISA Academic Article uri icon

abstract

  • The microalga Parietochloris incisa (Trebouxiophyceae, Chlorophyta) is the richest plant source of the polyunsaturated arachidonic acid (AA). Under nitrogen starvation, AA constitutes almost 60% of total fatty acids, reaching a content of over 20% of dry weight. Sequence analysis of the chloroplast Small-subunit (SSU) 16S rRNA and the nuclear SSU 18S rRNA genes showed the close similarity of this alga to several Chlorella species. Based on the phylogenetic similarity, we attempted to identify and clone for the first time the fatty acid desaturase genes of P. incisa, utilizing the sequences of low-temperature inducible Chlorella vulgaris gene for ω3 desaturase (Suga et al., 2002).

publication date

  • June 1, 2004