T-cell Responses Targeting HIV Env V2 in Natural Infection: Implications for RV144 Vaccine Recipients Academic Article uri icon

abstract

  • Background: The RV144 HIV-1 vaccine trial showed 31% efficacy, with Env V1/V2-specific antibodies inversely correlated with infection risk and viral genetic signatures of immune selection in the V1/V2 of breakthrough viruses. A sieve analysis to detect vaccine-induced, T- cell mediated viral escape showed that the selection could be partly attributed to T cells and that an HLA allele may have modified vaccine efficacy. Studies suggest vaccine-induced CD4+ T-cells preferentially targeted V2. Due to the high genetic variability, responses to V2 have not been adequately assessed in vaccine recipients, nor in HIV infection. Accurate quantification of V2-specific T-cell breadth and depth requires a unique peptide library. Methods: We devised an algorithm to design peptide libraries for mapping T cell epitopes in variable regions. Optimal peptides are selected for HLA binding to a frequency-weighted …

publication date

  • October 1, 2014