- Nano-sculptured thin films (nSTFs) is a group of meterials prepared by the oblique or the glancing angle deposition technique. They take the form of rods having different shapes such as nanocolumns, nanoscrews, nanozigzags and many other nanoshapes. Their potential for biosensing is highlighted in this review particularly the metallic ones due to their remarkable plasmonic properties. The techniques that have been shown so far to be of high potential are: extended surface plasmon resonance (SPR), localised SPR, surface enhanced flourescence (SEF) and Raman scattering (SERS). The use of metal nSTFs in SPR biosensors with Kretschmann-Raether configuration enhances both the angular and the spectral sensitivities due to the porosity and adds more degrees of freedom in designing evanescent waves based techniques. The metallic nSTFs, exhibit remarkable localised plasmonic properties that make them a promising substrate for enhanced spectroscopies. Their long term stability in water environment makes them suitable candidates for biosensing in water as it is already demonstrated for several water pollutants. The influences of the nanostructures' size, topology, the substrate features, and the preparation conditions on the enhancement of SEF and SERS are highlighted with emphases on the unresolved issues and future trends.