Glutamate diffusion in brain: a dual probe microdialysis study Academic Article uri icon


  • Materials and Methods: After approval of the Animal Care Committee, 8 male Sprague Dawley rats weighing 200–300 g were anesthetized with intraperitoneal urethane, rats were implanted with two microdialysis probes inserted in the striatum. Artificial CSF was perfused at a rate of 2 μl/min through the two probes for a duration of 60 min. A 1 M solution of glutamate was then perfused through the delivery probe, at a rate of 2 μl/min for the entire duration of the experiment while perfusing artificial CSF at the same rate of 2 μl/min through a recovery probe, located at 1 mm from the delivery probe. Aliqouots of 40 μl were collected from the recovery probe every 20 min. IV G injection performed at t= 100 min for 30 min (30 μmoles/min/100 g). The recovered G was measured using a spectrofluorometric assay (Graham and Aprison). Value are presented as mean±standard deviation. Results: Figure …

publication date

  • January 1, 2006