Inflorescence bud protease (s) against dehydrin-like inflorescence bud proteins of Pistacia vera Academic Article uri icon

abstract

  • This paper reports on a proteolytic activity extracted from Pistacia vera L.(pistachio) inflorescence buds, found in male and female tissues. The proteolytic activity reaches a peak at the beginning of bud opening and flowering and is directed against inflorescence bud dehydrin-like proteins of pistachio, IBP32 and IBP27. The effect of protease inhibitors indicated that the protease (s) belong to a serine-protease-like family and are not cysteine, acid or metallo-proteases. The proteolytic activity was strongly inhibited by Nα-p-tosyl-L- lysine chloromethyl ketone and to a lesser extent by N-tosyl-L-phenylalanine chloromethyl ketone, suggesting mainly trypsin-like specificity or broader serine-protease specificity. It is suggested that the proteolytic activity is important in the mobilization of nitrogen reserves stored in the bud storage proteins during dormancy to support the fast-developing …

publication date

  • January 1, 1999